An apertureless near-field microscope for fluorescence imaging
نویسندگان
چکیده
We describe an apertureless near field microscope for imaging fluorescent samples. Optical contrast is generated by exploiting fluorescent quenching near a metallized atomic force microscope tip. This microscope has been used to image fluorescent latex beads with subdiffraction limit resolution. The use of fluorescence allows us to prove that the contrast mechanism is indeed spectroscopic in origin. © 2000 American Institute of Physics. @S0003-6951~00!00403-4#
منابع مشابه
Imaging Information in DNA
Single molecule imaging with optical methods has become an important tool in biophysical studies. However, when imaging molecules at room temperature using far field optics, one can only resolve molecules that are separated by a distance greater than the diffraction limit ofthe microscope, about 220 nanometers. Near field techniques have allowed researchers to image with resolutions on the orde...
متن کاملApertureless near-field/far-field CW two-photon microscope for biological and material imaging and spectroscopic applications.
We present the development of a versatile spectroscopic imaging tool to allow for imaging with single-molecule sensitivity and high spatial resolution. The microscope allows for near-field and subdiffraction-limited far-field imaging by integrating a shear-force microscope on top of a custom inverted microscope design. The instrument has the ability to image in ambient conditions with optical r...
متن کاملAn apertureless near-field scanning optical microscope for imaging surface plasmons in the mid-wave infrared
ABSTRACT An apertureless near-field scanning optical microscope (a-NSOM) setup is described. Special consideration is given to important system components. Surface plasmons are defined, as is their relationship to aNSOM and their interaction with the scanning probe tip. We used this set-up to measure a metal-dielectric-metal (MDM) antenna integrated with a quantum cascade laser (QCL). The forme...
متن کاملScanning near-field ellipsometric microscope-imaging ellipsometry with a lateral resolution in nanometer range
An apertureless optical near-field scanning microscope system has been created by combining a commercially available atomic force microscope and an ellipsometer without any prior changes in design of the respective devices. In preliminary experiments, an optical resolution of about 20 nm ~l/32! has been achieved using the combined microscope. The intensity of the measured optical signal has bee...
متن کاملMeasurement and calculation of the near field of a terahertz apertureless scanning optical microscope
We present measurements and calculations of the terahertz (THz) electric field measured in the near field of a metal tip used in THz apertureless near-field optical microscopy (THz-ANSOM). An analytical model in which we treat the metal tip as a linear wire antenna allows us to predict almost all of the features observed in the measurements, such as the relatively slow decay of the near-field a...
متن کامل